Journal: Cells

Article Title: A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion

doi: 10.3390/cells10112855

Figure Lengend Snippet: Illustration of Microfluidic Flip-Chip ( A ) Image shows the fabricated MFC using a soft lithography process along with the illustration. ( B ) A graphic illustration of the MFC shows the three-layered structure with PDMS channels as the top layer, through-hole membrane as the middle layer, and titanium electrodes as the third bottom layer. ( C ) The parameters affecting chip performance—the PDMS membrane thickness (t m ), the diameter of fusion well (d w ), the distance between adjacent wells (d aw ), the distance between electrodes (d), and the distance between adjacent electrodes (d ae ) are as shown. Scale bar: 200 µm.

Article Snippet: COMSOL Multiphysics simulations of microfluidic channels, Figure S3.

Techniques: Membrane

Journal: Biofabrication

Article Title: Microfluidics-Based Laser Guided Cell-Micropatterning System

doi: 10.1088/1758-5082/6/3/035025

Figure Lengend Snippet: Diagram of the microfluidics-based laser guided cell-micropatterning system.

Article Snippet: A COMSOL multiphysics simulation of the flow rate through the microfluidic channel layer ( ) was conducted to determine the exit velocity of cell-suspensions from the microchannels. shows the simulation results based on the rectangular dimensions described in Section 2.2.1.

Techniques:

Journal: Biofabrication

Article Title: Microfluidics-Based Laser Guided Cell-Micropatterning System

doi: 10.1088/1758-5082/6/3/035025

Figure Lengend Snippet: An exploded assembly and overall diagram of the microfluidics-based cell-delivery biochip and cell-culture substrate.

Article Snippet: A COMSOL multiphysics simulation of the flow rate through the microfluidic channel layer ( ) was conducted to determine the exit velocity of cell-suspensions from the microchannels. shows the simulation results based on the rectangular dimensions described in Section 2.2.1.

Techniques: Cell Culture

Journal: Biofabrication

Article Title: Microfluidics-Based Laser Guided Cell-Micropatterning System

doi: 10.1088/1758-5082/6/3/035025

Figure Lengend Snippet: (A) 2D schematic of the microfluidic biochip flow-channel layer (depth is 50 μm). (B) Phase contrast image (20×) of a microchannel cross-section. Scale bar 50 μm.

Article Snippet: A COMSOL multiphysics simulation of the flow rate through the microfluidic channel layer ( ) was conducted to determine the exit velocity of cell-suspensions from the microchannels. shows the simulation results based on the rectangular dimensions described in Section 2.2.1.

Techniques:

Journal: Biofabrication

Article Title: Microfluidics-Based Laser Guided Cell-Micropatterning System

doi: 10.1088/1758-5082/6/3/035025

Figure Lengend Snippet: A single CFN at 16 hours: It was selected from the microfluidic cell-delivery channel and laser-micropatterned onto a PDMS-based cell-culture substrate.

Article Snippet: A COMSOL multiphysics simulation of the flow rate through the microfluidic channel layer ( ) was conducted to determine the exit velocity of cell-suspensions from the microchannels. shows the simulation results based on the rectangular dimensions described in Section 2.2.1.

Techniques: Cell Culture

Journal: Biofabrication

Article Title: Microfluidics-Based Laser Guided Cell-Micropatterning System

doi: 10.1088/1758-5082/6/3/035025

Figure Lengend Snippet: COMSOL simulation for the flow rate of cell-suspensions through the microfluidic biochip.

Article Snippet: A COMSOL multiphysics simulation of the flow rate through the microfluidic channel layer ( ) was conducted to determine the exit velocity of cell-suspensions from the microchannels. shows the simulation results based on the rectangular dimensions described in Section 2.2.1.

Techniques:

Journal: Biofabrication

Article Title: Microfluidics-Based Laser Guided Cell-Micropatterning System

doi: 10.1088/1758-5082/6/3/035025

Figure Lengend Snippet: Fluorescence and phase contrast combined image (40x) of a laser-micropatterned CFN array. The two DiI live-stained cells (red) were from one microfluidics-cell-delivery channel, the remainders were from the other channel. Scale bar 25 μm.

Article Snippet: A COMSOL multiphysics simulation of the flow rate through the microfluidic channel layer ( ) was conducted to determine the exit velocity of cell-suspensions from the microchannels. shows the simulation results based on the rectangular dimensions described in Section 2.2.1.

Techniques: Fluorescence, Staining